SubtiBank SubtiBank
xtmB [2020-04-21 14:33:55]
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xtmB [2020-04-21 14:33:55]

PBSX terminase (large subunit)
locus
BSU_12580
pI
8.87
mw
50.99 kDa
protein length
433 aa Sequence Blast
gene length
1302 bp Sequence Blast
function
phage DNA replication
product
PBSX terminase (large subunit)
essential
no
synonyms
ykxG
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.1|Prophages] → [category|SW 5.1.1|PBSX prophage]

    • Gene

    Coordinates
    1,325,890 1,327,191

    The protein

    Structure

  • [PDB|4IFE] (from Shigella phage, corresponds to aa 6 ... 225, 27% identity)

    • Expression and Regulation

    Operons

    genes
    [gene|DD4F52FE386EF75009ECF594ED4E2D1593220E4F|xtmA]-[gene|277F51EB4FE2BAB0FD1808EDE25705E10833AD0A|xtmB]-[gene|E77DD93E3CDCA9B81106E6AB0F18513360AAE9DB|xkdE]-[gene|6542BE447872C1A16C056DA21BD7A23C65F5C543|xkdF]-[gene|38173C76C27A7178CEE66E3642533EB34828C2CB|xkdG]-[gene|81C6812565B3E8ADCB19F52BDFFA9E55B94A2E9D|xkdH]-[gene|5ACCC439AD8849355094F8C5FC6EF29A9077EF58|xkdI]-[gene|6DF19E4546509687CA194F6B44EEB723C9467B9C|xkdJ]-[gene|31B1832E5EAD957EE6A5C7CB0C117EC0F26AA8AB|xkdK]-[gene|683D350D98AC0D27951145AD87830B89C0B4F393|xkdM]-[gene|0BF8AD1ACD0BBA6913636081B41E6D04D16D1BA2|xkdN]-[gene|156A8B699B32B268EBA1A0A1620C95D0DF630A4E|xkzB]-[gene|CD97483876F4FCCE451AD4F2A8AFD6F560074769|xkdO]-[gene|ED35852FAAD386CFB43F8D3DDEF28F7B05EC8B43|xkdP]-[gene|D5FDCC792D0E6B8D35BCDDC0D7B76025ACC900FF|xkdQ]-[gene|AF9706F2EBF056A404B5D7640D78D1E833805225|xkdR]-[gene|C222F247C225FD6DEBB103DFB169AEF87DFC0C0D|xkdS]-[gene|1234C9BC164FB47E003009E943124982260221C0|xkdT]-[gene|BDE032590D5F3504CDB4D6BD545153D681B65ADD|xkdU]-[gene|3BCF7587145CFEDEA2F36512ABF62D6B6A2886C5|xkzA]-[gene|9FAAA2E63B161862396C47CE5D1EA9191847FDAD|xkdV]-[gene|1F152F97AD20DABAC1D83EDC2177F46EAECC562C|xkdW]-[gene|787561F433911D090F801AD493F688895498542F|xkdX]-[gene|27BB6659541BA5368DEDB19EADCD372CE41244F5|xepA]-[gene|630FC0D9BDD243D6BF94DBDE506EC2A9CB0AAFE6|xhlA]-[gene|360D866E939DDB822AFAC589D563B191A88695CD|xhlB]-[gene|50D7B97E4D3D41F74EF1942B85DE1DA4A33BEBB0|xlyA]
    description
    [Pubmed|22383849,2110147]

    sigma factors

  • [protein|458406A4E6824C24493CFC19718F10720AA3B453|Xpf]: sigma factor, [Pubmed|8083174], in [regulon|458406A4E6824C24493CFC19718F10720AA3B453|Xpf regulon]

    • regulation

  • expression during spore [SW|germination] is strongly reduced under conditions of osmotic stress [Pubmed|27766092]
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    Biological materials

    Mutant

  • BKE12580 ([gene|277F51EB4FE2BAB0FD1808EDE25705E10833AD0A|xtmB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGAGGGTTGATTTCTTTTA, downstream forward: _UP4_CGAGAAAGGAGGAGGTCATA
  • BKK12580 ([gene|277F51EB4FE2BAB0FD1808EDE25705E10833AD0A|xtmB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12580 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATGAGGGTTGATTTCTTTTA, downstream forward: _UP4_CGAGAAAGGAGGAGGTCATA

    • References

  • 2110147,8083174